杨爱芳教授博士生导师Tel:(86)531-88364350E-mail:nihaohua@263.net教育背景AifangYangProfessorofCellBiologyandGeneticsSchoolofLifeScience(86)531-88364350(phone)ShandongUniversity(86)531-88564350(FAX)Jinan,250100,P.R.Chinanihaohua@263.net(E-mail)EDUCATIONPh.D.ShandongUniversity,December2003(DevelopmentalBiology)Dissertationtitle:“Breedingsalt-tolerantmaterialswitheliteagronomictraitsforBetavulgarisL.viabioengineeringtechniques”.M.S.ShandongUniversity,September1996(CellBiology)Thesistitle:“ChangesofCa2+andsolubleproteinsringthededifferentiationofculturedcellsofLinumusitatissimumL.”.B.S.ShandongUniversity,September1993(Botany)Thesistitle:“Thechangesofphenolcomplexesringthecultureofcottoncalli”.EMPLOYMENT2007.11-2008.12VisitingResearchScholar,PlantBiology&PathologyDepartment,RutgersUniversity（StateUniversityofNewJersey）2006-current,Professor,InstituteofPlantCell&Genetics,SchoolofLifeScience,ShandongUniversity2001-2006,AssociateProfessor,InstituteofPlantCell&Genetics,SchoolofLifeScience,ShandongUniversity1998-2001,Instructor,DepartmentofBiochemistry,SchoolofLifeScience,ShandongUniversity1996-1998,TeachingAssistant,DepartmentofBiochemistry,SchoolofLifeScience,ShandongUniversityRESEARCHEMPHASISGeneCloningandMolecularBreedingInthepastyears,Ihadbeenengagedinplantbiotechnologyresearchesandaccumulatedplentyexperiencesintissueculture,plantletregeneration,somaclonalvariantselection,geneidentificationandgenetictransformationofcropsincludingmaize,beet,wheatandcotton;andGramineaefoliagesofperennialryegrass(LoliumperenneL.),annualryegrass(LoliummultiflorumLam.),commonorchardgrass(DactylisglomerataL.),tallfescue(FestucaarundinaceaSchreb.),Kentuckybluegrass(PoapratensisL.),Lawngrass(ZoysiajaponicaSteud)andsoon.WehadestablishedandoptimizedAgrobacterium-mediatedtransformationprotocolsusingeithercalliormultiplebudsorshootsinvitrooreventhenakedmeristemofshoottipsinvivo,bypassingtheinvitrocultureprocess,asrecipients,andgenesinvolvedinabioticstressresistance,sugarorstarchmetabolicpathwaysordevelopmentandnutritionutilizationandsoonsuchasbetA,AtNHX1,DREB1A,DREB2A,PIS,PPase,1-fft,1-sst,galE,lgtC,etc,clonedmainlyfromsaltcress,maize,lettuceandyeasts,respectively,weretransferredintothesematerialsandtransgenicplantsandprogenieswithmodifiedcharacterswereobtained,respectively,tomeettheagriculturalorhorticulturalneed.Moreover,thetransgeneexpressionprofile,hereditystabilityintheprogeniesandtheeffectsofthetransgeneexpressiononthetransgenicplantscharacterswerealsooneofmymajors.PlantBiologicalReactorComparedtootherbiologicalreactors,likemicrobesandanimalcellexpressionsystems,plantshaveuniqueadvantages.Therefore,transgenicplantsasanewbiologicalreactorpossesgreatpotentials.Usingplanttoprocebiologicallyactiveprocts,includingvaluableproteins,carbohydratesforclinical,foodandinstrialpurposesisthegoalofourresearch.Modificationofthecarbohydratemetabolicpathwaysinplantsusinggeneticapproachesmighttransformplantsintobiologicalreactorstoprocevaluableoligo-,poly-saccharideandglycoconjugates.Geneinvolvedincarbohydratemetabolicpathwaysandinstarchsynthesispathwayshavebeenidentifiedandtransferredintotobacco，beetandmaize,respectively,andinspiringresultshavebeenobtained.Grants1.AFYang,JRZhang,KPLietal.Creationofnewmaizegermplasmwithefficientphosphorus-utilizationtraitsviageneticmanipulation(2008AAZX08003-005),fundedbyMinistryofAgricultureofthePeople’sRepublicofChina,￥4,200,000,2008-2010.2.AFYang,JRZhang,KWZhang,XFGuetal.Creationofnewgermplasmforbreedingofsupermaize(2007BAD31B01),fundedbyMinistryofScience&TechnologyofthePeople’sRepublicofChina,￥6,000,000,2007-2010.3.AFYang,JRZhang,XFGuetal.Screeningandplantingofsea-waterresistantvarietiesinthebeachofyellowriverdelta(2007AA091701),HighTech-ResearchandDevelopmentProgram(863)ofChina,fundedbyMinistryofScience&TechnologyofthePeople’sRepublicofChina,￥700,000,2007-2010.4.JurenZhang,AifangYang,KeweiZhangetal.Functionalgenomicsofstress-,desease-resistanceandseedqualityinmaize(2007AA10A107).HighTech-ResearchandDevelopmentProgram(863)ofChina,fundedbyMinistryofScience&TechnologyofthePeople’sRepublicofChina,￥研究成果及发表论文Publications2008:1.CRWang,AFYang,GDYue,QGao,HYYin,JRZhang.EnhancedexpressionofphospholipaseC1(ZmPLC1)improvesdroughttoleranceintransgenicmaize.Planta,2008,227:1127-1140.SCI.IF3.062.CRWang,AFYang,HYYin,JRZhang.InfluenceofWaterStressonEndogenousHormoneContentsandCellDamageofMaizeSeedlings.JournalofIntegrativePlantBiology,2008,50(4):427-434.SCI.IF0.673.LZWang,AFYang,CMHe,MLQu,JRZhang.CreationofnewmaizegermplasmusingalienintrogressionfromZeamaysssp.exicana.Euphytica,2008,164:789-8014.KPLi,CZXu,ZXLi,KWZhang,AFYang,JRZhang.Comparativeproteomeanalysesofphosphorusresponsesinmaize(ZeamaysL.)rootsofwild-typeandlow-PtolerancemutantrevealrootcharacteristicsassociatedwithP-efficiency.ThePlantJournal,2008,55：927-939.SCI,IF6.755.ZHSui,LYNiu,GDYue,AFYang,JRZhang,Cloningandexpressionanalysisofsomegenesinvolvedinthephosphoinositideandphospholipidsignalingpathwaysfrommaize(ZeamaysL.).Gene,2008,doi:10.1016/j.gene.2008.09.004.SCI,IF.2.872007:6.KPLi,CZXu,KWZhang,AFYang,JRZhang*.Proteomicanalysisofrootsgrowthandmetabolicchangesunderphosphorusdefecitinmaize(ZeamaysL.)plants.Proteomics,2007,2007,7(9):1501-1512.7.SLLv,AFYang,KWZhang,LWang,JRZhang*.Increaseofglycinebetainesynthesisimprovesdroughttoleranceincotton.MolecularBreeding,2007,20:233-248.8.XGDuan,AFYang,FGao,SLZhang,JRZhang*.HeterologousexpressingvacuolarH+-PPaseenhancedelectrochemicalgradientacrossvacuolarmembraneandimprovedtobaccocellsalttolerance.Protoplasm,2007,232(1-2):87-959.KPLi,ZPXug,KWZhang,AFYang,JRZhang.Efficientproctionandcharacrerizationformaizeinbredlineswithlow-phosphorustolerance.PlantScience,2007,172:255-264.10.AFYang,KWZhang,XYYin,JRZhang*.Analysisofagronomictraitsandcombiningabilityoftransgenicplantsinmaize.ScientiaAgriculturaSinica,2007,40(12):2895-2902(inChinese).11.HJLi,AFYang,XCZhang,FGao,JRZhang.Improvingfreezingtoleranceoftransgenictobaccoexpressingsucrose:sucrose1-fructosyltransferasegenefromLactucasativa.PlantCell,Tissue,OrganCulture,2007,2007,89:37-48.2006:12.FGao,QGao,XGDuan,GDYue,AFYang*,JRZhang.CloningofanH+-PPasegenefromThellungiellahalophilaanditsheterologousexpressiontoimprovesalt-toleranceoftobacco.JournalofExperimentalBotany,2006,57(12):3256-3270.13.AFYang,CMHe,KWZhang,JRZhang*.ImprovementofAgrobacterium-mediatedtransformationofembryogeniccallifrommaizeeliteinbred.InVitroCellularandDevelopmentBiology-Plant,2006,42(3):215-219.14.AFYang,SLZhao,XYYin,FGao,JRZhang.IntroctionofAtNHX1intobeetimprovedsalt-toleranceoftransgenicplants.HighTechnologyLetters,2006,12(2):182-187.2005:15.AFYang,XGDuan,XFGu,FGao,JRZhang*.Efficienttransformationofbeet(BetavulgarisL.)andproctionofplantswithimprovedsalt-tolerance.PlantCell,Tissue,OrganCulture,2005,83:259-270.16.XRHu,AFYang,KWZhang,JWang,JRZhhang*.OptimizationofinVitroMultipleShootClumpInctionandPlantletRegenerationofKentuckybluegrass(PoapratensisL.).PlantCell,Tissue,OrganCulture,2005,83:342-350.17.XFGu,AFYang,HMeng,JRZhang.Invitroinctionoftetraploidplantsfromdiploidzhanhuawinterjujube(ZizyphusJujubaMill.).PlantCellReports,2005,24:671-676.18.YSZhang,SMZhai,AFYang,JRZhang.Maizetransformationviapollentubepathwayandtheinheritanceoftransgeneinprogeny.HighTechnologyLetters,2005,11(2):202-206.(inChinese).19.SMZhai,ZHSui,AFYang,JRZhang.Characterizationofanovelphosphoinositide-specificphospholipaseCfromZeamaysanditsexpressioninEscherichiacoli.BiotechnologyLetters,2005,27:799-804.20.YSZhang,XYYin,AFYang,GSLi,JRZhang*.Stabilityofinheritanceoftransgenesinmaize(ZeamaysL.)linesprocedusingdifferenttransformationmethods.Euphytica,2005,144:11-22.21.FGao,QGao,GDYue,AFYang,JRZhang*.CloningofCBF1genefromThellungiellasalsuginea.JournalofShandongUniversity,2005,40:113-120.(inChinese).22.HZhang,XGDuan,AFYang*,JRZhang.OptimizationoftransformationconditionsinshootclumpsofBetamaritimaL.mediatedbyAgrobacteriumtumefaciens.SugarCropsofChina,2005,1:1-5.(inChinese).23.HZhang,XGDuan,AFYang*,JRZhang.GenetictransformationofBetamaritimaL.byAgrobacteriumtumefaciensandproctionofsalttoleranttransgenicplants.HighTechnologyLetters,2005,15(8):72-77.(inChinese).24.AFYang,SYJiang,XRHu,XGDuan,JRZhang*.EfficientinvitropropagationandsalttolerantplantletregenerationinRumex.ActaPrataculturaeSinica,2005,14(2):43-47.(inChinese)25.SYJiang,XRHu,XGChao,AFYang*.EfficientproctionofmultipleshootsandinflorescencesinctionfromshootofloliummultiflorumL.andloliumperenneL.ActaPrataculturaeSinica,2005,14(6):100-106.(inChinese)26.XRChao,LLSong,XRiHu,SYJiang,AFYang*.Highefficiencyinctionofmultipleshootsfromorchardgrass(DactylisglomerataL.)andregenerationofsalt-tolerantplantlets.ShandongAgriculturalScience,2005,1:7-10.(inChinese)2004:27.AFYangetal.Inctionofmultiplebudclumpsfrominflorescencetipsandregenerationofsalt-tolerantplantletsinBetavulgarisL.PlantCell,Tissue,OrganCulture,2004,77:29-34.28.XYYin,AFYang,KWZhang,JRZhang.Proctionandanalysisoftransgenicmaizewithimprovedsalt-tolerancebyintroctionofAtNHX1gene.ActaBotanicaSinica,2004,46(7):854-861.29.SYJiang,AFYang,XR,JRZhang.TissuecultureandplantletregenerationofRumex.PlantPhysiologyCommunication,2004,40(1):61.(inChinese)30.AFYang,CMHe,XLWang,JRZhang.Invitrocultureandplantregenerationfromimmatureinflorescencesofperennialryegrass（LoliumperenneL.）andItalianryegrass（LoliummultiflorumLam.）varieties.ActaPrataculturaeSinica,2004,13(5):84-90.(inChinese)31.CMHe,XLWang,SYJiang,XRHu,AFYang.InctionofcallifromyoungearsandimmatureembryosofRyeDongmu70andregenerationofplantlets.ActaPrataculturaeSinica,2004,13(6):(inChinese)32.HZhang,AFYang,XGDuan,JRZhang.HighefficientinctionofmultiplebudclumpsandregenerationofplantletsforBetamaritimaL.SugarCropsofChina,2004,1:1-4.(inChinese)2003:33.AFYang,LPZhu,XGDuan,Fgao,JRZhang.TransformationofsugarbeetwithbetAgeneimprovedsalt-toleranceoftransgenicplants.Collectionofthesisofhigh-techforumofmarineorganisms,2003,923-933.(inChinese)34.XYWang,XYShan,AFYang,JRZhang.Constructionofplantexpressionvectorandanalysisofherbicide-resistanceandsalt-toleranceoftransgenictobacco.JournalofAgriculturalBiotechnology.2003,11(6):554-560.(inChinese)2002:35.AFYang,SLZhao,LPZhu,SMZhai,JRZhang,YueHuang,XiaolingZhai.Thecreationofnewsugarbeetgermplasmtoleranttosaltbygenetictransformation.ShandongAgriculturalScience,2002(2):3-6.(inChinese)2001:36.LPZhu,AFYang,JRZhang,KSChen.Multipleshootinctionandplantletregenerationinsugarbeet.HighTechnologyLetters,2001,11(125):17-20.(inChinese)37.GSLi,AFYang,JRZhang,YPBi,LShan.Genetictransformationofcallifrommaizeandregenerationofherbicide-resistantplantlets.ChineseScienceBulletin,2000,45：2181-2184(inChinese);2001,46(7):563-566(inEnglish).2000:38.XHLi,DLLi,JRnZhang,AFYang.SomaticembryogenesisandplantregenerationinhighfrequenciesfromculturedshootapicesofZeamays.PlantPhysiologyCommunications,2000,36(5):430-433.(inChinese)39.TGLi,AFYang,KWZhang,JRZhang.SelectionofmutantsresistanttoFusariumgraminearumandstudiesontheprogeniesoftheregeneratedplants.ScientiaAgriculturaSinica,2000,33(supplement):147-151.(inChinese)1999:40.XFWang,AFYang,JRZhang.GenetictransformationofprotoplastsfromPeucedanumterebinthaceumandregenerationofberbicide-resistantplantlets.ActaBotanicaSinica,1999,41(7):706-710.(inChinese)1998:41.JRZhang,SFGao,AFYang,JJYu.SelectionofresistantmutantsforHelminthosporiummaydisviatissuecultureinmaize.ChineseJournalofBiotechnology,1998,14(4):456-459.(inChinese)Patents:1.AFYang,JRZhang,KWZhang,HFWang.Amethodforcreatingnewsalt-toleantgermplasmthroughvariationandselectionofbeetmultiplebuds.Chinesepatent,ZL02135731.5,2004.2.AFYang,JRZhang,XYYin,KWZhang,XFGu.Amethodanditsutilizationforestablishingarecipientsystemforgenetictransformationofryegrass.Chinesepatent,ZL200410024550.3,2006.3.JRZhang,AFYang,KWZhang,XYYin,XFGu.AmethodanditsutilizationforestablishingagenetictransformingsystemforKentukybluegrass.Chinesepatent,ZL200410024537.8,2007.4.JRZhang,MShang,XHLi,AFYang,QWZhangi.Amethodanditsutilazationfortransformingplantswithmacroseeds.Chinesepatent,ZL01104428.4,2005.5.JRZhang,AFYang,XFGu,KWZhang,XYYin.Amethodforimprovingsalt-anddrought-toleranceofmaizeandwheatviapyramidingbetA、NHX1、Ppasegene.Chinesepatent,ZL200510044791.9,2007.*如果发现导师信息存在错误或者偏差，欢迎随时与我们联系，以便进行更新完善。联系方式>>

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