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杨春玉教育背景:时间学校学位1994-1998山东农业大学学士1999-2002山东大学硕士2005-2009山东大学博士工作经历:时
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盛多红,博士,山东大学微生物技术国家重点实验室研究人员。联系方式:地址:山东济南山大南路27号,250100电话:13658612032邮箱:dhsheng@s
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卢丽丽博士,副教授电话:0531-88365128E-mail:lililu@s.e.cn教育经历1998.9~2002.6,湖北师范学院,生物科学学士;20
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钟耀华副教授博士,硕士生导师E-mail:zhongyaohua@s.e.cnPhone:0531-88366118;13853149665Fax:0531-88366
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赵小凡教授博士生导师电话;0531-88364620E-mail:xfzhao@s.e.cn 教育背景从事昆虫生理生化与分子生物学研究,重点研究昆虫发育过程中蜕皮与变态的功能基因和分子调控机理。承担本科生和研究生教学工作。1990-1991年赴日本从事家蚕半胱氨酸蛋白酶研究;1998-2000年获德国政府学术交流会(DAAD)“生物科学特别项目博士后奖学金”赴德国从事烟草天蛾V-ATP酶研究;2001-2002年在日本九州工业大学从事DNA和蛋白质测序、基因重组蛋白包涵体复性和纯化、毕赤酵母表达外源基因等研究;2003年获德国政府德意志联合研究会(DFG)资助赴德国从事基因重组病毒杀虫剂构建和蛋白酶、HHR3在昆虫细胞中的表达等研究。2005年赴日本从事蛋白质组学研究。2006年赴美国密苏里大学进行短期科研工作。先后承担国家自然科学基金重点项目、重大国际合作项目和面上项目;参加国家“973”和“863”项目;以及承担山东省自然科学基金重点项目等科研项目10余项。主要学术成果有:从棉铃虫鉴定并纯化到组织蛋白酶B,克隆到该蛋白酶基因(HCB),阐明了该蛋白酶在棉铃虫生长发育中的功能,从大豆等植物种子中鉴定并纯化到该蛋白酶的抑制因子,对肿瘤细胞有抑制生长的作用;克隆了棉铃虫蜕皮调节转录因子HHR3基因并阐明了该基因的表达和激素调控模式;阐明了多种棉铃虫幼虫蜕皮和变态时期差异表达的蛋白质和基因,为研究昆虫发育的分子机制提供了新靶标;证明了非甾醇类蜕皮激素竞争物(RH-2485)可以导致棉铃虫致死性提前蜕皮,可用于田间控制棉铃虫;构建了HCB和HHR3基因重组核型多角体病毒,可用于开发高技术生物杀虫剂用于害虫控制。获得国家发明专利多项。济南市第十二届、第十三届、第十四届人大代表。 研究方向研究生招生专业:生化与分子生物学研究方向:昆虫生化与分子生物学研究内容:以棉铃虫为材料,用分子生物学方法研究昆虫蜕皮与变态的分子机理,包括:蜕皮激素信号转导途径、蜕皮与变态功能基因的表达与调控、变态发育中组织重建的分子机理、血细胞在变态发育中的功能等。Researchinterest:Weusethecottonbollworm(Helicoverpaarmigera)asamodeltoinvestigatethemechanismofinsectmoltingandmetamorphosisbymolecularmethods.Recentstudiesinclude:20-OH-ecdysteroidhormonesignaltransctionpathway;expressionandregulationofmoltingandmetamorphosisrelatedgenes;molecularmechanismoftissueremodeling;andfunctionofhaemocytesinmoltingandmetamorphosis.在研课题1.昆虫血细胞在变态中的功能和作用机理,国家自然科学基金。2.害虫暴发成灾的遗传与行为机理,国家重大基础研究项目973。已完成课题14.棉铃虫皮层溶离中蛋白酶表达及调控研究,国家自然科学基金,2007.1-2009.12。项目负责人。13.重大农业害虫猖獗危害的机制及可持续控制的基础研究——害虫生长发育与生殖调控的分子机制。国家重大基础研究项目(973)。2006.1-2010.12。参加。12.家蚕重要经济性状功能基因克隆,国家高技术研究发展计划(863计划)2006.1-2010.12。参加。11.昆虫蜕皮激素信号转导途径研究,国家自然科学基金重大国际合作项目,2008.1-2011.12月。项目负责人。10.棉铃虫蜕皮级联反应功能基因表达研究,国家自然科学基金重点项目,2004.1-2007.12。项目负责人。9.基因重组病毒杀虫剂研究,山东省自然科学基金重点项目,2004.1-2006.12.项目负责人。8.重组病毒杀虫剂的研制,863,国家科技部,2001-2004.项目负责人。7.棉铃虫蜕皮调节转录因子基因克隆和表达载体构建,国家自然科学基金,2001.1-2003.1.项目负责人。6.棉铃虫卵蛋白酶基因克隆及功能表达,国家自然科学基金,1999.1-2001.12.项目负责人。5.RH-化合物对棉铃虫的防治效果研究,山东省科委国际合作基金,1998.1-2000.12.项目负责人。4.蜕皮激素类似物合成及对棉铃虫的防治研究,山东省科委优秀年轻教师奖励基金,1999.1-2000.12.项目负责人。3.棉铃虫卵内蛋白酶纯化,国家自然科学基金主任基金,1998.1-12.项目负责人。2.鳞翅目昆虫卵内蛋白酶纯化性质与cDNA克隆.国家教育部优秀年轻教师基金,1996.1-1998.12.项目负责人。1.蓖麻蚕卵黄蛋白质降解酶研究,国家教育部留学回国人员基金,1992.1-1994.12.项目负责人。研究成果及发表论文代表性论文:*为通讯作者,下划线为并列作者。63.WangChuan-Xu,ZhengWei-Wei,LiuPeng-Cheng,WangJin-Xing,ZhaoXiao-Fan*,2012,Thesteroidhormone20-hydroxyecdysoneupregulatedtheproteinphosphatase6fortheprogrammedcelldeathintheinsectmidgut.AminoAcids.DOI10.1007/s00726-011-1159-9.62.DongDu-Juan,LiuPeng-Cheng,WangJin-Xing,Xiao-FanZhao*,2012,TheknockdownofHa-GRIM-19byRNAinterferenceincedprogrammedcelldeath.AminoAcids.42(4):1297-307.61.HeHong-Juan,HouLi,WangJin-Xing,ZhaoXiao-Fan*,2012,Theapoptosisinhibitorsurvivinpreventsinsectmidgutfromcelldeathringpostembryonicdevelopment.MolecularBiologyReport.39(2):1691-9.60.ZhangFeng-Xia,ShaoHong-Lian,WangJin-Xing,ZhaoXiao-Fan*,2011,b-thymosinisupregulatedbythesteroidhormone20-hydroxyecdysoneandmicroorganisms.InsectMolecularBiology.20,519–527.59.LiuPeng-Cheng,WangJin-Xing,SongQi-Sheng,ZhaoXiao-Fan*,2011,Theparticipationofcalponininthecrosstalkbetween20-Hydroxyecdysoneandjuvenilehormonesignalingpathwaysbyphosphorylationvariation.PLoSONE.6:e19776.58.WangGang,LiuPeng-Cheng,WangJin-Xing,ZhaoXiao-Fan*,2011,ABTBdomain-containinggeneisupregulatedbyimmunechallenge.ArchivesofInsectBiochemistryandPhysiology.77:58-71.57.YangXiao-Hui,LiuPeng-Cheng,ZhengWei-Wei,WangJin-Xing,ZhaoXiao-Fan*,2011,Thejuvenilehormoneanaloguemethopreneup-regulatestheHa-RNA-bindingprotein.MolecularandCellularEndocrinology333:172–180.56.HouLi,WangJin-Xing,ZhaoXiao-Fan*,2010,Rab32andtheremodelingoftheimaginalmidgutinHelicoverpaarmigera.AminoAcids,DOI10.1007/s00726-010-0720-2.55.WangQian,LiuYang,HeHongJuan,ZhaoXiao-Fan*,Jin-XingWang*,2010,ImmuneresponseofHelicoverpaarmigeratodifferentkindsofpathogens.BMCImmunology.11:9.54.WangJia-Lin,WangLi-fu,WangJin-Xing,ZhaoXiao-Fan*,2010,Theexpressionpatternsofaeukaryoticinitiationfactor3subunithinthesilkglandsinBombyxmori.Archivesofinsectbiochemistryandphysiology.75:1-12.53.HeHong-Juan,WangQian,ZhengWei-Wei,WangJin-Xing,SongQi-Sheng,ZhaoXiao-Fan*,2010,FunctionofNuclearTransportFactor2andRaninthe20EsignaltransctionpathwayintheCottonBollworm,Helicoverpaarmigera.BMCCellBiology.11:1.52.WangLi-Fu,ChaiLian-Qin,HeHong-Juan,WangQian,WangJin-Xing,ZhaoXiao-Fan*,2010,ThecathepsinL-likeproteinaseisinvolvedinmoltingandmetamorphosisinHelicoverpaarmigera.InsectMolecularBiology,19:99-111.51.ZhengWei-Wei,YangDan-Tong,WangJin-Xing,SongQi-Sheng,GilbertLawrenceI.,ZhaoXiao-Fan*,2010,Hsc70bindstoultraspiracleresultingintheupregulationof20-hydroxyecdsone-responsivegenesinHelicoverpaarmigera.MolecularandCellularEndocrinology.315:282-291.50.FuQiang,LiuPeng-Cheng,WangJin-Xing,SongQi-Sheng,ZhaoXiao-Fan*,2009,Proteomicidentificationofdifferentiallyexpressedandphosphorylatedproteinsinepidermisinvolvedinlarval-pupalmetamorphosisofHelicoverpaarmigera.BMCGenomics.10:600.49.LiuYang,SuiYi-Peng,WangJin-XingandZhaoXiao-Fan*,2009,Characterizationofthetrypsin-likeprotease(ha-tlp2)constitutivelyexpressedintheintegumentofthecottonbollworm,Helicoverpaarmigera.ArchivesofInsectBiochemistryandPhysiology.72:74-87.48.SuiYi-Peng,WangJin-XingandZhaoXiao-Fan*,2009,Theimpactsofclassicalinsecthormonesontheexpressionprofilesofanewdigestivetrypsin-likeprotease(TLP)fromthecottonbollworm,Helicoverpaarmigera.InsectMolecularBiology.18:443-452.47.SuiYi-Peng,LiuXiao-Bin,ChaiLian-Qin,WangJin-Xing,ZhaoXiao-Fan*,2009,CharacterizationandinfluencesofclassicalinsecthormonesontheexpressionprofilesofamoltingcarboxypeptidaseAfromthecottonbollworm(Helicoverpaarmigera).InsectMolecularBiology,18:353–363.46.DongDu-Juan,WangJin-Xing,ZhaoXiao-Fan*.2009,Aeukaryoticinitiationfactor5Cisupregulatedringmetamorphosisinthecottonbollworm,Helicoverpaarmigera.BMCDevelopmentalBiology.9:19.45.WangJia-Lin,ZhangYa-Ping,GuYin-Yu,WangJin-XingandZhaoXiao-Fan*,2009,FunctionofaTGF-inciblenuclearproteininthesilkglandinBombyxmori.InsectMolecularBiology.18:243–251.44.TianYuan-Yuan,LiuYang,ZhaoXiao-Fan*,WangJin-Xing*,2009,CharacterizationofaC-typelectinfromthecottonbollworm,Helicoverpaarmigera.DevelopmentalandComparativeImmunology.33:772–779.43.ShaoHong-Lian,ZhengWei-Wei,LiuPeng-Cheng,WangQian,WangJin-Xing,ZhaoXiao-Fan*,2008,EstablishmentofaNewCellLinefromLepidopteranEpidermisandHormonalRegulationontheGenes.PLoSONE3:e3127.42.SuiYi-Peng,WangJin-XingandZhaoXiao-Fan*,2008,Effectsofclassicalinsecthormonesontheexpressionprofilesofalipasegenefromthecottonbollworm(Helicoverpaarmigera).InsectMolecularBiology.17:523–529.41.ShaoHong-Lian,DongDu-Juan,HuJin-Dong,WangJin-Xin,ZhaoXiao-Fan*,2008,ConstructionoftherecombinantbaculovirusAcMNPVwithcathepsinB-likeproteinaseanditsinsecticidalactivityagainstHelicoverpaarmigera.PesticideBiochemistryandPhysiology.91:141-161.40.WangJia-Lin,WangJin-Xing,ZhaoXiao-Fan*,2008,MolecularCloningandExpressionProfilesoftheAcyl-CoA-BindingProteinGeneFromtheCottonBollwormHelicoverpaarmigera.ArchivesofInsectBiochemistryandPhysiology.68:79–88.39.ChaiLian-Qin,TianYuan-Yuan,YangDan-Tong,WangJin-Xing,ZhaoXiao-Fan*,2008,MolecularcloningandcharacterizationofaC-typelectinfromthecottonbollworm,Helicoverpaarmigera.Developmental&ComparativeImmunology,32:71-83.38.YangDan-Tong,ChaiLian-Qin,WangJin-Xing,ZhaoXiao-Fan*,2008,MolecularcloningandcharacterizationofHearmcaspase-1fromHelicoverpaarmigera.MolecularBiologyReport.35:405-12.37.YangXiao-Mei,HouLi-Jing,WangJin-Xing,ZhaoXiao-Fan*,2007,ExpressionandFunctionofCathepsinB-LikeProteinaseinLarvalHemocytesofHelicorverpaarmigeraringMetamorphosis.ArchivesofInsectBiochemistryandPhysiology.64:164–174.36.HuJindong,ShaoHonglian,ZhangYubao,FuQiang,SunChen,WangJinxing,ZhaoXiaofan*,2007,ConstructionofHaSNPVwithHelicoverpahormonereceptor3.HighTechnologyLetters,3:327-331.35.ShaoHong-Lian,DongDu-Juan,HuJin-Dong,ZhangXin-Chang,ZhangYu-Bao,FuQiang,SunChen,WangJin-Xin,ZhaoXiao-Fan*,2007,ReconstructionofAcMNPVwithHelicoverpahormonereceptor3anditseffectontheHelicoverpalarvae.BiocontrolScienceandTechnology.17:95-104.34.DongDu-Juan1,HeHong-Juan1,ChaiLian-Qin1,JiangXiao-Juan,WangJin-Xing,ZhaoXiao-Fan*,2007,IdentificationofgenesdifferentiallyexpressedringlarvalmoltingandmetamorphosisofHelicoverpaarmigera.BMCDevelopmentalBiology,7:73.33.WangJia-Lin,JiangXiao-Juan,WangQian,HouLi–Jing,XuDa-Wei,WangJin-Xing,ZhaoXiao-Fan*,2007,IdentificationandexpressionprofileofaputativebasementmembraneproteingeneinthemidgutofHelicoverpaarmigera.BMCDevelopmentalBiology,7:76.32.ZhangXin-Chang,ShaoHong-Lian,WangJin-Xing,ZhaoXiao-Fan*,2006,PurificationofaninhibitorofHelicoverpacathepsinB-likeproteinasefromsunflowerseed.JournalofEnzymeInhibitionandMedicinalChemistry,21:433–439.31.YangXiao-Mei,HouLi-Jing,DongDu-Juan,ShaoHong-Lian,WangJin-Xing,ZhaoXiao-Fan*,2006,CathepsinB-LikeProteinaseIsInvolvedintheDecompositionoftheAltFatBodyofHelicoverpaarmigera.ArchivesofInsectBiochemistryandPhysiology,62:1-10.30.ZhaoXiao-Fan*,HeHong-Juan,DongDu-Juan,WangJin-Xing.2006,IdentificationofDifferentiallyExpressedProteinsringLarvalMoltingofHelicoverpaarmigera.JournalofProteomeResearch,5:164-169.29.ZhaoXiao-Fan*,AnXiao-Meng,WangJin-Xing,DongDu-Juan,DuXin-Jun,SuedaS.andKondoH.,2005,ExpressionofHelicoverpacathepsinB-likeproteinaseringembryonicdevelopment,ArchivesofInsectBiochemistryandPhysiology,58:39-46.28.ZhaoXiao-Fan*,WangJin-Xing,LiFei-Xue,SuedaShinji,KondoHiroki.2005,AnalysisofsubstratespecificityandendopeptidylcleavagesitesofthecathepsinB-likeproteinasefromHelicoverpaarmigera,TheProteinJournal,24:219–225.27.ZhaoXiao-fan*,WangJin-Xing,XuXia-Lian,LiZhao-Min,KangCui-Jie,2004,Molecularcloningandexpressionpatternsofmolt-regulatingtranscriptionfactor-hhr3fromHelicoverpaarmigera.InsectMolecularBiology.13:407-412.26.ZhaoXiaofan*,LiZhaomin,WangJinxing,WangJiabao,WangShengli,BlankespoorD.Harvey,2003,EfficacyofRH-2485,anewnon-steroidalecdysoneagonist,againstcottonbollworm,Helicoverpaarmigera(lepidoptera:noctuidae)inlaboratoryandfield.CropProtection.22:959-965.25.ZhaoX-F*,WangJ-X,XuX-L,SchmidR.,WieczorekH.,2002,MolecularcloningandcharacterizationofthecathepsinB-likeproteinasefromthecottonbollworm,Helicoverpaarmigera.InsectMolecularBiology.11:567-575.24.MerzendorferH.,ReinekeS.,ZhaoX-F,JacobmeierB.,HarveyW.R.,WieczorekH.,2000,ThemultigenefamilyofthetobaccohornwormV-ATPase:novelsubunitsa,C,D,H,andputativeisoforms.BiochemicaetBiophysicaActa.1467:369-379.23.ZhaoXiao-Fan*,WangJin-Xing,WangYin-Chang,1998,Purificationandcharacterizationofacysteineproteinasefromeggsofthecottonbollworm,Helicoverpaarmigera.InsectBiochemistryandMolecularBiology.28:259-264.22.ZhaoXiaofan,WangJinxing,NorikoYagi.,YamamotoYoshimi,WatabeShoji,TakahashiY.Sussum,1996,OccurrenceofacathepsinB-likeacidcysteinproteinaseintheeggsofsilkwormmoth,Antheraeapernyi.ComparativeBiochemistryandPhysiology.113B:95-103.21.TakahashiS.Y.,YamamotoY.,ZhaoX-F,WatabeS.,1996,Bombyxacidcysteineproteinase.InvertebrateReproctionandDevelopment.30:265-281.20.YamamotoY.,ZhaoX-F,SuzukiA.C.,Takahashi.SY.,1994,CysteineproteinasefromtheeggsoftheSilkworm,Bombyxmori:siteofsynthesisandasuggestedroleinyolkproteindegradation.JournalofInsectPhysiology.40:447-454.19.TakahashiS.Y.,ZhaoX-F,KageyamaT.,YamamotoY.,1992,Acidcysteineproteinasefromtheeggsofsilkmoth,Bombyxmori:tissuedistribution,developmentalchangesandthesitesofsynthesisfortheenzyme.InsectBiochemistryandMolecularBiology.22:369-377.18.赵小凡,2007,昆虫蜕皮的分子机理及应用。昆虫知识,44:323-326。17.郑薇薇,张玉宝,王金星,赵小凡*,2007,棉铃虫蜕皮时期同工酶表达模式。昆虫学报,50:317-322。16.董杜鹃扈进冬张新昌李子劲王金星赵小凡*2006,HCB在Pichia中的表达及鉴定。生物工程杂志,26:44-48。15.赵小凡,基因组时代的昆虫学。2005,3:26-27。14.张新昌,刘杨,王金星,赵小凡*,2005,从大豆中纯化棉铃虫组织蛋白酶B抑制因子HCB-SoyI。昆虫学报,48:849-853。13.杨晓梅,侯立静,董杜鹃,王金星,赵小凡*,2005,组织蛋白酶B在棉铃虫发育过程中的表达及活性研究。山东大学学报.40:119-124。12.邵红莲,赵小凡*,董杜鹃,扈进冬,邵丁丁,杨晓梅,王金星,2005,棉铃虫组织蛋白酶B在杆状病毒表达系统中的表达。山东大学学报,40:107-111。11、杨晓梅,侯立静,康翠洁,邵红莲,张新昌,杜欣军,王金星,赵小凡*,2004,棉铃虫组织蛋白酶B单克隆抗体的制备及鉴定。山东大学学报,39:116-120。10.安晓萌,杜欣军,邵红莲,邵丁丁,赵小凡*,王金星,2004,棉铃虫组织蛋白酶B多克隆抗体制备及鉴定。昆虫知识,41:132-126。9.杜欣军,邵丁丁,赵小凡*,王金星,2004,棉铃虫组织蛋白酶B在大肠杆菌中的表达。农业生物技术学报,12:162-166。8.赵小凡*,蒋晓娟,杜欣军,徐筠娉,王金星,2004,棉铃虫蜕皮调节转录因子在大肠杆菌中的重组表达及包涵体纯化。昆虫学报,47:281-286。7.李照民,赵小凡*,王金星,李斐雪,王家宝,王胜利,2003,非甾醇类蜕皮激素竞争物RH-2485对棉铃虫蛋白质组成和表皮结构的影响。动物学研究,24:140-143。6.张志宏,赵小凡*,王金星,2002,棉铃虫卵内半胱氨酸蛋白酶的分离纯化、氨基酸序列分析及蛋白酶鉴定。昆虫学报,45:840-843。5.张志宏,王金星,赵小凡*,王来元,2002,棉铃虫雌性成虫脂肪体cDNA文库的构建。山东大学学报,37:449-451。4.李照民,赵小凡*,李斐雪,王金星,2002,RH-2485对棉铃虫实验室防治效果实验。植物保护学报,29:78-82。3.徐夏莲,赵小凡*,王金星,2001,棉铃虫组织蛋白酶B组织分布与合成部位研究。动物学研究,22:242-245。2.李斐雪,李照民,张志宏,徐夏莲,赵小凡*,王金星,2001,棉铃虫半胱氨酸蛋白酶底物特异性研究。农业生物技术学报,9:152-155。1.赵小凡,2001,昆虫学研究热点述评。《国际学术动态》,2:51-53。*如果发现导师信息存在错误或者偏差,欢迎随时与我们联系,以便进行更新完善。联系方式>>
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赵建教授博士生导师电话:0531-88364690E-mail:zhaojian@s.e.cn教育背景赵建,男,1970年出生,博士,教授,博士生导师(200
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张玉忠教授博士生导师Tel:0531-88564326E-mail:zhangyz@s.e.cn教育背景学习和工作经历:1985年9月—19
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张玉臻教授博士生导师电话:0531-88362258E-mail:yzzhang@s.e.cn教育背景张玉臻,女,生于1949年2月,教授,1997年起任博士生导
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张伟教授博士生导师电话:0531-88364332E-mail:weizhang@s.e.cn教育背景2009年6月-教授博士生导师山东大学生命科学学